Invitrogen™

Flp-In™ Complete System

Our Flp-In™ Complete System allows integration and expression of your gene of interest in mammalian cells at a specific genomic자세히 알아보기

Our Flp-In™ Complete System allows integration and expression of your gene of interest in mammalian cells at a specific genomic location. The Flp-In System involves introduction of a Flp Recombination Target (FRT) site into the genome of the mammalian cell line of choice. An expression vector containing your gene of interest is then integrated into the genome via Flp recombinase-mediated DNA recombination at the FRT site.

The major components of the Flp-In™ System include:

• A Flp-In. target site vector, pFRT⁄lacZeo, for generation of a host cell line containing an integrated FRT site
• An expression plasmid containing a FRT site linked to the hygromycin resistance gene for Flp recombinase-mediated integration and selection of a stable cell line expressing your gene of interest under the control of the human cytomegalovirus (CMV) immediate-early enhancer⁄promoter
• A Flp recombinase expression plasmid, pOG44, for expression of the Flp recombinase under the control of the human CMV promoter
• A control expression plasmid containing the chloramphenicol acetyl transferase (CAT) gene, which when cotransfected with pOG44 into your Flp-In. host cell line, expresses CAT

사양

구성 또는 유도성 시스템Constitutive

배달 유형Transfection

프로모터CMV

제품 유형Flp-In Complete System

선택 제제(진핵)Zeocin™, Hygromycin

단백질 태그Untagged

클로닝 방법Restriction Enzyme/MCS

수량1 kit

벡터pFRT

제품라인Flp-In™, pcDNA™

용도(애플리케이션)Stable Cell Line Development, Targeted Integration

Unit SizeEach