Thermo Scientific™

EZ-Link™ TFP Ester-PEG12-DBCO

EZ-Link TFP Ester-PEG12-DBCO is a hetero-bifunctional crosslinker that contains an amine-reactive tetrafluorophenyl (TFP) group and a dibenzylcyclooctyne (DBCO) group with자세히 알아보기

EZ-Link TFP Ester-PEG12-DBCO is a hetero-bifunctional crosslinker that contains an amine-reactive tetrafluorophenyl (TFP) group and a dibenzylcyclooctyne (DBCO) group with a water-solubilizing long PEG12 spacer in between. This crosslinker can be used to label any primary-amine-containing molecule (such as a protein) with a distantly attached DBCO group. Once a protein or (bio)molecule is DBCO-labeled, it can undergo a copper-free azide-alkyne cyclo-addition reaction to form a stable triazole linkage with an azide-labeled coupling partner.

EZ-Link TFP Ester-PEG12-DBCO features include:
• Bioorthogonal—DBCO and azides react selectively to form triazole linkages
• Heterobifunctional—reactive towards amine and azide
• Soluble—reagent readily dissolves in water-miscible solvents for dilution into aqueous reaction mixtures
• Stable—TFP esters are more hydrolytically stable than NHS esters

EZ-Link TFP Ester-PEG12-DBCO can be used to create diverse conjugates by means of the bioorthogonal coupling between an alkyne and azide (supplied separately). The coupling or crosslinking procedure is typically done in two steps. First, a protein- or amine-containing molecule is labeled with a DBCO group and excess crosslinker is removed. Second, an azide-containing moiety (prepared independently) is introduced into the same system as the DBCO moiety and labeling occurs under biological conditions without the introduction of any additional reagents such as a copper catalyst.

Classic click reactions involve a copper-catalyzed azide-alkyne cyclo­addition to label or conjugate molecules. A drawback of this approach is that copper ions can impair protein function, harm cells, and reduce the fluorescence of fluorophores. DBCO is distinct in that it doesn`t require copper to catalyze the reaction with an azide for triazole formation. The strain in the eight-membered ring allows the reaction with azide-modified molecules to occur in the absence of catalysts, preventing copper-induced damage to cells or proteins.

사양

화학물질 반응성Amine-Azide

CleavableNo

설명TFP Ester-PEG12-DBCO

분자량1067.12

페길레이티드Yes

스페이서 암 길이46.3 Å

세포 투과성Not determined

배송 조건Wet Ice

제품라인EZ-Link™

라벨링 방법Chemical Labeling

교차결합제 유형Heterobifunctional

반응성 부분TFP Ester, DBCO

스페이서Long (>30 Å)

형태Liquid

수량25 mg

용해도DMF, DMSO

형식Standard

수용성No

제품 유형Crosslinker

Unit SizeEach