Thermo Fisher Scientific D4S234E Polyclonal Antibody, Biotin
D4S234E 단백질을 인식하는 Biotin-conjugated rabbit polyclonal antibody로, Western blot, IHC, ELISA, IP에 적합합니다. 인간, 마우스, 랫트 시료에 반응하며, 신경세포 내 수용체 재활용 연구에 유용합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 140–190 on human Neuron-specific protein family member 1 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
NEEP21 (Neuron-enriched endosomal 21kDa protein), also known as brain neuron cytoplasmic protein 1, NSG1 (neuron-specific proteins family member 1), P21, or D4S234E, is a single-pass type II membrane protein belonging to the NSG family. It is highly expressed during neuronal maturation but is downregulated in adult tissues.
NEEP21 localizes predominantly to Rab4-positive early endosomes in the somatodendritic neuronal compartment and is essential for proper receptor sorting and recycling in neurons. It associates with GRIP1 and GluR-2 to mediate surface expression of GluR-2. Disruption of this interaction leads to GluR-2 accumulation in early endosomes and altered synaptic current properties. Additionally, NEEP21 forms a complex with the SNARE protein Syntaxin 13 (Syntaxin 12) and participates in recycling of transferrin receptors (TFRs) and neurotensin receptor 2 (NTR2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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