
Thermo Fisher Scientific ARHE Monoclonal Antibody (5C7B6)
ARHE 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry, ELISA에 사용 가능. 정제된 재조합 인간 RND3 단백질(AA 104-241)을 면역원으로 사용. Protein G로 정제되어 PBS 내 0.05% sodium azide 포함. 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5C7B6 |
| Immunogen | Purified recombinant fragment of human RND3 (AA: 104–241) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at −20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787520 |
Product Specific Information
MA5-31897 has been tested in indirect ELISA.
Target Information
Members of the Rho family of Ras-related GTPases, such as ARHE, regulate the organization of the actin cytoskeleton in response to extracellular growth factors. Like Ras, Rho family members appear to cycle between an inactive GDP-bound form and an active GTP-bound form. Three major regulators of Rho activity have been identified:
- RhoGDIs: interact with GDP-bound Rho proteins to maintain a resting complex
- GEFs: promote GDP/GTP exchange leading to activation
- GAPs: stimulate GTP hydrolysis to return the protein to its inactive form
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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