Thermo Fisher Scientific 5T4 Polyclonal Antibody
Thermo Fisher Scientific의 5T4 Polyclonal Antibody는 Mouse 5T4 단백질을 표적으로 하는 Sheep 유래 IgG 항체입니다. Western Blot, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합하며, 높은 특이성과 순도를 제공합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 5–15 µg/mL | - |
| Flow Cytometry (Flow) | 2.5 µg per million cells | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse 5T4 (Ser30–Ser361) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2576257 |
Product Specific Information
- In direct ELISAs, approximately 5% cross-reactivity with recombinant human 5T4 is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
This gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. The encoded protein is an oncofetal antigen specific to trophoblast cells. In adults, it is highly expressed in many tumor cells and is associated with poor clinical outcomes in numerous cancers. Alternate splicing in the 5′ UTR results in multiple transcript variants encoding the same protein.
(provided by RefSeq, Oct 2009)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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