Thermo Fisher Scientific 5T4 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5-15 µg/mL

-

Flow Cytometry (Flow)

2.5 µg per million cells

-

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse 5T4 Ser30-Ser361 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:5T4,uniProtId:Q9Z0L0-1,ncbiNodeId:10090,antigenRange:30-361,antigenLength:426,antigenImageFileName:PA5-47690_5T4_Q9Z0L0-1_Sheep.svg,antigenImageFileNamePDP:PA5-47690_5T4_Q9Z0L0-1_Sheep_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2576257

Product Specific Information

In direct ELISAs, approximately 5% cross-reactivity with recombinant human 5T4 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

This gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. The encoded protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is associated with poor clinical outcome in numerous cancers. Alternate splicing in the 5` UTR results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2009].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.