Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 550
DyLight 550 형광 표지된 염소 유래 항-마우스 IgG(H+L) 2차 항체로, WB, IHC, ICC, Flow 등 다양한 응용에 적합합니다. 교차 흡착 처리로 비특이적 반응을 최소화하며, 높은 신호 대비와 재현성을 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Mouse IgG-heavy and light chain |
| Conjugate | DyLight™ 550 |
| Excitation/Emission Max | 556 / 570 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556753 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, horse, human, pig, rabbit, and rat |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to bovine, chicken, horse, human, pig, rabbit, and rat.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful in detection, sorting, or purification of the specified target.
Secondary antibodies offer increased versatility by enabling the use of multiple detection systems (e.g., HRP, AP, fluorescence) and greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats Available
Various conjugate formats of Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody are available, including Alexa Fluor™, DyLight™, FITC, HRP, Biotin, and others. Custom conjugation requests are also supported.
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