Thermo Fisher Scientific EGFRvIII Recombinant Rabbit Monoclonal Antibody (RM419)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

-

Flow Cytometry (Flow)

1:10-1:100

-

ELISA (ELISA)

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

RM419

Immunogen

A peptide corresponding to epidermal growth factor receptor variant III (EGFRvIII) (aa 1-100). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EGFRvIII,uniProtId:P00533-1,ncbiNodeId:9606,antigenRange:1-100,antigenLength:1210,antigenImageFileName:MA5-36216_EGFRvIII_P00533-1_Rabbit.svg,antigenImageFileNamePDP:MA5-36216_EGFRvIII_P00533-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.02 mg/mL

Purification

Protein A

Storage buffer

PBS with 50% glycerol, 1% BSA

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2884050

Product Specific Information

MA5-36216 reacts to human Epidermal Growth Factor Receptor Variant III. No cross reactivity with wild type EGF receptor.

Target Information

EGFRvIII is included in receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.