Thermo Fisher Scientific F4/80 Monoclonal Antibody (BM8), Biotin, eBioscience
Mouse F4/80 항원을 인식하는 BM8 클론의 Biotin 결합 단클론 항체로, 성숙 대식세포 표지에 사용됩니다. Flow cytometry 등 다양한 응용에 적합하며, 0.25 µg/test로 권장됩니다. 4°C 보관, 동결 금지.
- 카탈로그번호
- 13-4801-8x (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific F4/80 Monoclonal Antibody (BM8), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 146 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 9 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 2 |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 11 |
| Immunocytochemistry (ICC/IF) | - | 44 |
| Flow Cytometry (Flow) | 0.25 µg/test | 221 |
| Neutralization (Neu) | - | 1 |
| Functional Assay (Functional) | - | 1 |
| Miscellaneous PubMed (Misc) | - | 10 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Hamster, Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BM8 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466656 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC, APC-eFluor 780
- Brilliant UV 395, 563, 661, 805
- Brilliant Violet 421, 650, 711
- eFluor 450, 506, 570, 660
- FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, Super Bright 436, 600, 645, 702, 780
- Functional Grade
- Request custom conjugation
Product Specific Information
The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 160 kDa surface receptor belonging to the EGF-TM7 family of proteins. It contains seven EGF-like domains, seven transmembrane sequences, and a cytoplasmic tail homologous to other TM7 family members.
F4/80 is expressed on most mature macrophages and serves as a key marker for this population. Other cell types, such as peritoneal eosinophils, Langerhans cells, and certain dendritic cell subsets, may also express F4/80. Expression begins early in myeloid development and can be upregulated by M-CSF stimulation in vitro. Some macrophage subsets, particularly in lymphoid tissues, may lack F4/80 expression.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes and bone marrow cells; ≤0.25 µg/test recommended. One test corresponds to staining in a 100 µL final volume (10⁵–10⁸ cells/test). Optimize antibody concentration for specific assays.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
F4/80 antigen is a 160 kDa cell surface glycoprotein, part of the EGF-TM7 family, sharing 68% amino acid identity with human EMR1 (EGF module-containing mucin-like hormone receptor 1). Expression varies with macrophage maturation and activation, and is found on Kupffer cells, Langerhans cells, microglia, peritoneal, pulmonary, thymic, and splenic red pulp macrophages. It may also be present on some dendritic cell subsets but is absent in macrophages of T cell zones.
Although ligands and biological roles are not fully defined, F4/80 is suggested to contribute to efferent CD8⁺ regulatory T cell generation.
For Research Use Only. Not for diagnostic use or resale without authorization.
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