Invitrogen™

Chargeswitch™ Pro Filter Plasmid Midi Kit

The Chargeswitch™ -Pro Filter Plasmid Midi Kit includes 25 plasmid midipreps and provides high yield and high purity plasmid with자세히 알아보기

The Chargeswitch™ -Pro Filter Plasmid Midi Kit includes 25 plasmid midipreps and provides high yield and high purity plasmid with the fastest and the simplest protocol.

The ChargeSwitch™-Pro Plasmid Filter Mini, Midi, Maxi Kits generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications. The system is based upon a novel dual⁄nested column design which facilitate the fastest and simplest protocol for high quality plasmid. The system is composed of two columns. The inner flat bottomed column, also referred to as “lysate clarification” column, contains filter materials suitable for rapid clarification of bacterial lysate. The outer luer bottomed column, also referred to as “binding” column, contains ChargeSwitch™ (CST) derivatized membrane that immediately binds plasmid DNA from the clarified lysate. This nested-column design enables simultaneous clarification and DNA binding in a single short centrifugation (or vacuum) step. The combined advantages of novel, optimized plastics and a unique chemistry are an extreme competitive advantage of speed and convenience.

To accomplish this, the ChargeSwitch™-Pro Kit provides:

• 100% ethanol-free, guanidinium-free, organics-free, aqueous protocol for a safer, more streamlined method
• Cleaner purified product for improved performance in downstream applications
• A novel Dual⁄nested column design for simultaneous lysate clearing and binding step which offers the fastest and the simplest protocol
• Endotoxin units less than 10 EU ⁄ ug DNA for
• Vacuum Elution can be done with the EveryPrep™ Universal Vacuum Manifold (SKU # K211101)

How it works
The ChargeSwitch™ technology replaces the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification with a simple ion-exchange mechanism. At low pH values (less than 6.5) the ChargeSwitch™ surface ligand is positively charged and binds plasmid DNA. Raising the pH (>8.5) neutralizes the charge and elutes the bound nucleic acid. The ChargeSwitch™ protocol retains the ease and familiarity of the common silica spin column protocol with a binding, washing, and elution step, making adapting to the ChargeSwitch™ method effortless.

사양

컬럼 유형Spin Column

형식Spin Column

Isolation TechnologyChargeSwitch Chemistry

스케일Midi

샘플 종류Bacterial Culture

용리량0.5 to 1 mL

최종 제품 유형Plasmid DNA

용도(애플리케이션)Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription

고처리량 호환성Not High-throughput Compatible (Manual)

포함Columns with Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer

프렙 스케일100-200 μg (Medium-Scale) Plasmid DNA

제품라인ChargeSwitch™

제품 유형Pro Filter Plasmid Midi Kit

수량25 preps

순도Transfection Grade

배송 조건Room Temperature

유통 기한Guaranteed stable for 6 months when properly stored.

타겟Plasmid DNA

시스템 유형ChargeSwitch™

테스트 시간30 min.

수율200 μg

시작 물질 양25 ml

Unit SizeEach