Thermo Fisher Scientific TRIM5 alpha/gamma/delta Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

3 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (ASGILVNVKEEVTC) corresponding to the N-terminus amino acids of TRIM5 (aa 2-15). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TRIM5 alpha/gamma/delta,uniProtId:Q9C035-1,ncbiNodeId:9606,antigenRange:2-15,antigenLength:493,antigenImageFileName:PA5-18156_TRIM5_alphagammadelta_Q9C035-1_Goat.svg,antigenImageFileNamePDP:PA5-18156_TRIM5_alphagammadelta_Q9C035-1_Goat_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_10974151

Product Specific Information

This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.

Target Information

TRIM5a is a 493 amino acid member of the large tripartite motif protein (TRIM) family. TRIM proteins are composed of three zinc-binding domains, a RING, a B-box 2 and a coiled-coil domain, and they use homomultimerization to identify different cell compartments. Some TRIM proteins, such as TRIM5a, also possess a carboxy-terminal B30.2 (SPRY) domain and localize to the cytoplasm. TRIM5a mediates innate intracellular retroviral resistance, which is dependent on its carboxy-terminal domain. The three variable regions of the B30.2 domain form loops on one side of the B30.2 core structure of TRIM5a which may form a binding surface for the virus. TRIM5a trimerization plays a major role in its affinity for the retroviral capsid, and in its ability to inhibit virus infection. The linker region between the coiled-coil and B30.2 domains of TRIM5a is required for this trimerization. TRIM5a blocks infection after the virus has entered the cell.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.