Gibco™

Expi293F™ Inducible Cells

The Expi293F Inducible cell line is derived from engineered Expi293F cells that stably express the tetracycline repressor (TetR) protein, thus자세히 알아보기

The Expi293F Inducible cell line is derived from engineered Expi293F cells that stably express the tetracycline repressor (TetR) protein, thus enabling tetracycline-regulated protein expression. The cell line was established by the stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter. It is a powerful tool for tetracycline-inducible high-yield expression of recombinant proteins.

Features of Expi293 Inducible cells include:
• Suspension, high-density culture in Expi293 Expression Medium
• Tetracycline-inducible protein expression with extremely low basal expression levels
• Equivalent protein yields to parental Expi293F cells—up to 1 g/L
• Significantly higher protein yields than other HEK293 Tet-inducible cell line alternatives

For inducible expression of your protein of interest, pcDNA5/TO mammalian expression vector (Cat. No. V103320) or an equivalent inducible vector is required for tetracycline-regulated gene expression.

Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in Expi293 Expression Medium and 10% DMSO. The cells should be thawed directly into Expi293 Expression Medium.

사양

제품라인Expi293™

수량1 vial

배송 조건Dry Ice

Unit SizeEach