Thermo Fisher Scientific Amphiphysin Polyclonal Antibody
Amphiphysin 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC(P), ICC/IF에 사용 가능. Human, Mouse, Rat에 반응하며, Affinity chromatography로 정제됨. PBS/glycerol buffer에 보관하며 -20°C에서 안정적 저장 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human AMPH (Accession P49418), corresponding to amino acid residues S139–E189 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2850856 |
Product Specific Information
Antibody detects endogenous levels of total AMPH.
Target Information
Amphiphysins are members of the BAR (Bin-Amphiphysin-Rvsp) protein superfamily that play a key role in clathrin-mediated endocytosis of synaptic vesicles (SVs).
All members share a conserved N-terminal BAR domain and a C-terminal Src homology (SH3) domain.
Two isoforms of amphiphysin act together as a heterodimer, interacting via the SH3 domain with dynamin, synaptojanin, and clathrin.
These complexes are involved in synaptic vesicle recycling at nerve terminals.
Amphiphysins are also identified as the dominant autoantigen in paraneoplastic Stiff-Man Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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