Thermo Fisher Scientific F(ab`)2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
HRP 결합 F(ab`)2-Goat anti-Rabbit IgG (H+L) 2차 항체로, Western blot, IHC, ELISA에 적합. Rabbit IgG에 높은 특이성과 교차흡착 처리로 비특이적 결합 최소화. Lyophilized 형태로 안정적 보관 가능하며, 다양한 형광 및 효소 결합 포맷 제공.
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- A10547
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- Antibodies
- 판매단위
- pk
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Thermo Fisher Scientific F(ab`)2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:5,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| ELISA | 1:500–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rabbit |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_10561554 |
| Target | IgG |
| Cross Adsorption | Against pooled human serum, mouse serum, mouse plasmacytoma/hybridoma proteins and purified human paraproteins |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
Storage and reconstitution:
The formulation of this antibody was updated from PBS with 0.02% thimerosal to PBS without thimerosal on 31/Mar/2024. Any lots produced after this date will not contain thimerosal (lot numbers greater than 2855945).
Store product at -20°C until opened. Reconstitute using 0.5 mL PBS, pH 7.2, to yield a 1 mg/mL stock solution.
After reconstitution in buffer without a stabilizer, the product is stable for approximately three months at 4°C.
For prolonged storage after reconstitution without a stabilizer, add glycerol to a final concentration of 50% (v/v), aliquot, and store at -20°C (stable up to one year).
If reconstituted in buffer containing a preservative (e.g., PBS with 0.02% thimerosal), the product is stable for up to one year at 4°C.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins.
They are useful for detection, sorting, or purification of rabbit targets.
Secondary antibodies offer increased versatility with multiple detection systems (HRP, AP, fluorescence) and provide greater sensitivity through signal amplification.
Typically generated by immunizing a host animal with pooled rabbit immunoglobulins, followed by purification and modification (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to ensure high specificity.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
Available Conjugate Formats
- Alexa Fluor series (350, 405, 430, 488, 514, 532, 546, 555, 568, 594, 633, 635, 647, 660, 680, 700, 750, 790)
- Alexa Fluor Plus series (488, 555, 594, 647)
- AP, APC
- Biotin, Biotin-XX
- Cyanine3, Cyanine5
- DyLight series (350, 405, 488, 550, 594, 633, 650, 680, 755, 800)
- FITC, Pacific Blue, Pacific Orange
- PE, PE-Alexa Fluor 610, PE-Alexa Fluor 647
- Rhodamine, Rhodamine Red-X
- Texas Red, Texas Red-X, TRITC
- Custom conjugation available upon request
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