Thermo Scientific™

GeneBLAzer™ LXRβ-UAS-bla HEK 293T Cells

LXR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Liver-X receptor-beta (LXR beta) fused to the자세히 알아보기

LXR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Liver-X receptor-beta (LXR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-LXR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. LXR beta-UAS-bla HEK 293T 293 cells are functionally validated for Z` and EC50 concentrations of TO901317. In addition, cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

사양

대체 작용제DHT

길항제Cyp Acetate, Spirolactone, OHF

어세이 입력Cell-based beta lactamase reporter gene

셀 상태Dividing Cells

검출 방법Fluorescence

종Human

약효를 기대할 수 있는 타겟Nuclear Receptors

셀 수2 x 106 Cells

일차 작용제R1881

제품라인GeneBLAzer™

제품 유형HEK 293T Cells

수량2 x 106 cells

리포터 유전자BLA (Beta-Lactamase)

타겟 입력LXR beta, NR1H2, LXRB

Unit SizeEach