Thermo Fisher Scientific TOP1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DNA topoisomerase I (DNA topoisomerase I (TOP1)) (NP_0032771) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Topoisomerase I,uniProtId:P11387-1,ncbiNodeId:9606,antigenRange:1-100,antigenLength:765,antigenImageFileName:PA5-120171_Topoisomerase_I_P11387-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120171_Topoisomerase_I_P11387-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.17 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2913743

Product Specific Information

Positive test controls include: MCF7, SKOV3, SW480, Jurkat, HeLa, Mouse spleen, Mouse ovary . The target is usually found in the following locations: Nucleus, nucleolus, nucleoplasm.

Immunogen sequence: MSGDHLHNDS QIEADFRLND SHKHKDKHKD REHRHKEHKK EKDREKSKHS NSEHKDSEKK HKEKEKTKHK DGSSEKHKDK HKDRDKEKRK EEKVRASGDA

Target Information

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.