Thermo Fisher Scientific TOP1 Polyclonal Antibody
TOP1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ELISA, IP에 사용 가능. Human 및 Mouse 시료 반응. Affinity Chromatography로 정제되었으며, PBS/glycerol 버퍼에 보관. 핵 및 핵소체에서 주로 발현되는 TOP1 검출에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 1–100 of human DNA topoisomerase I (TOP1) (NP_003277.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.17 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913743 |
Product Specific Information
- Positive test controls: MCF7, SKOV3, SW480, Jurkat, HeLa, Mouse spleen, Mouse ovary
- Subcellular localization: Nucleus, nucleolus, nucleoplasm
Immunogen sequence:
MSGDHLHNDS QIEADFRLND SHKHKDKHKD REHRHKEHKK EKDREKSKHS NSEHKDSEKK HKEKEKTKHK DGSSEKHKDK HKDRDKEKRK EEKVRASGDA
Target Information
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. It catalyzes transient breaking and rejoining of a single DNA strand, allowing strand passage and topological change.
The gene is localized to chromosome 20 and has pseudogenes on chromosomes 1 and 22.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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