Thermo Fisher Scientific CD195 (CCR5) Monoclonal Antibody (NP-6G4), Alexa Fluor 488, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test
• Publications: View 2 publications

Product Specifications

Additional Formats

• APC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The NP-6G4 monoclonal antibody reacts with human CD195 (CCR5), an approximately 45 kDa member of the G protein-coupled receptor (GPCR) superfamily that binds chemokines including MIP-1α, MIP-1β, and RANTES. CCR5 is expressed on resting T cells (memory/effector phenotype), monocytes, macrophages, and immature dendritic cells. It functions as a receptor directing lymphocyte chemotaxis and serves as the primary co-receptor for macrophage-tropic HIV-1.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometry using normal human peripheral blood cells.

• Recommended staining: 37°C for 1 hour in Flow Cytometry Staining Buffer (Product #00-4222-57)
• Use at 5 µL (0.5 µg) per test (100 µL final volume)
• Suitable for 10⁵–10⁸ cells/test
• Excitation: 488 nm
• Emission: 519 nm
• Laser: Blue Laser

Target Information

Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, for infection. CCR5, along with CXCR4, CCR3, CCR2b, and CCR8, functions as an HIV coreceptor. CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains, facilitating viral fusion and entry into white blood cells. Beta-chemokines RANTES, MIP-α, and MIP-β are ligands for CCR5 and inhibit M-tropic HIV-1 infection. CCR5 mRNA is expressed in lymphoid organs and monocytes.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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