Thermo Fisher Scientific Mouse IgG1 Isotype Control

Applications

Tested Dilution

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Flow Cytometry (Flow)

10 µLx10^6 cells or 100 µL whole blood

Control (Ctrl)

Assay-Dependent

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Isotype Control

Immunogen

Rat thymocyte membrane glycoproteins

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539478

Product Specific Information

MA5-18095 is negative on all human cells and cell lines tested. This antibody recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species.

This product is a Low Endotoxin formulation, <0.01 EU/ug.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Target Information

Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.