Thermo Fisher Scientific CD366 (TIM3) Monoclonal Antibody (F38-2E2), PE, eBioscience

Thermo Fisher Scientific CD366 (TIM3) Monoclonal Antibody (F38-2E2), PE, eBioscience™

Applications

• Flow Cytometry (Flow)
  Tested dilution: 5 µL (0.125 µg)/test
  View 15 publications

Product Specifications

Available Formats

• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 805
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 786
• FITC
• Functional Grade
• PE-Cyanine5
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

This F38-2E2 monoclonal antibody reacts with human CD366, also known as T cell immunoglobulin and mucin domain-containing protein 3 (TIM3) or HAVCR2.
CD366 is expressed on activated CD4+ T cell subsets (Th1, Th17, Treg), CD8+ T cells, monocytes, dendritic cells, and mast cells.
Galectin-9 is identified as the ligand for CD366. In humans, this receptor negatively regulates CD4+ T cells, influencing Th1- and Th17-related cytokine secretion.
CD366 is implicated in tolerance, autoimmune disease (e.g., multiple sclerosis), and HIV infection.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and validated for flow cytometric analysis of stimulated normal human peripheral blood cells.
Use at 5 µL (0.125 µg) per test (defined as staining a 100 µL cell sample).
Cell number range: 10⁵–10⁸ cells/test.

Excitation: 488–561 nm
Emission: 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TIM3 (HAVCR2) is a 281 amino acid, type I Th1-specific cell surface glycoprotein expressed on terminally differentiated CD4+ Th1 and CD8+ Tc1 cells.
TIM3 contains an IgV-like domain, a mucin-like domain, and a conserved tyrosine phosphorylation motif.
It is involved in macrophage activation, autoimmune disease induction, and immune tolerance regulation.
Pathological significance includes experimental autoimmune encephalomyelitis (EAE) and Th1-mediated immune response modulation.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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