Thermo Fisher Scientific H4K5ac Polyclonal Antibody
Rabbit polyclonal antibody specific for acetylated Histone H4 at Lys5. Validated for WB, IHC, and ICC applications. Supplied in liquid form at 1 mg/mL concentration. For research use only, not for diagnostic procedures.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human Histone H4 (Accession P62805), corresponding to amino acid residues M1–I30 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2815895 |
Product Specific Information
Antibody detects endogenous levels of Acetyl-Histone H4 only when acetylated at Lys5.
Target Information
Histones are basic nuclear proteins responsible for nucleosome structure in eukaryotic chromatin. Each nucleosome consists of an octamer of core histones (H2A, H2B, H3, and H4) around which approximately 146 bp of DNA is wrapped. The linker histone H1 interacts with linker DNA between nucleosomes, aiding in chromatin compaction. The H4 gene is intronless, encodes a histone H4 family member, and contains a palindromic termination element instead of a polyA tail. It is located within the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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