
Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
HRP 결합된 Goat anti-Rabbit IgG (H+L) 2차 항체로 Western blot, IHC, ELISA, IP에 적합함. Rabbit IgG의 중쇄와 경쇄에 반응하며 높은 특이성과 민감도 제공. 항원 친화 크로마토그래피로 정제되어 안정적인 신호 증폭 가능.
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Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:200,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| ELISA | 1:5,000–1:10,000 |
| Immunoprecipitation (IP) | 1:500–1:5,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Purified Rabbit IgG, whole molecule |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 15 mg/mL BSA, 50 mM sucrose |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient |
| RRID | AB_228341 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
Product #31460 has been successfully used in Western blot, IHC, ELISA, and IP applications.
This antibody reacts with the heavy chains of rabbit IgG and the light chains common to most rabbit immunoglobulins, but does not react with non-immunoglobulin serum proteins. It may cross-react with immunoglobulins from other species and with SuperBlock™ Blocking Buffers.
Store at 4°C until opened. To extend shelf-life, add an equal volume of glycerol (final concentration ~50%) and store at -20°C.
Reconstitute with 2.0 mL of distilled water (final concentration 0.8 mg/mL).
Target Information
Anti-Rabbit secondary antibodies are affinity-purified reagents with well-characterized specificity for rabbit immunoglobulins. They are useful for detection, sorting, or purification of rabbit targets.
Secondary antibodies provide versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to one primary antibody.
They are typically generated by immunizing host animals with pooled rabbit immunoglobulins and purified by immunoaffinity chromatography.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Conjugate Formats
- Alexa Fluor 488-10 nm colloidal gold
- Alexa Fluor 488-5 nm colloidal gold
- AP
- Biotin
- DyLight 350
- DyLight 405
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 633
- DyLight 650
- DyLight 680
- DyLight 800 4X PEG
- FITC
- PE-Cyanine5.5
- TRITC
- WesternDot 625
- Custom conjugation available
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