Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), NovaFluor Yellow 660, eBioscience

Thermo Fisher Scientific CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), NovaFluor Yellow 660

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.05 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 615
• eFluor 450
• eFluor 506
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 555
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 760
• NovaFluor Red 710
• NovaFluor Yellow 570
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Custom conjugation available

Product Specific Information

The 53-2.1 monoclonal antibody reacts with mouse CD90.2 (Thy-1.2), a GPI-linked membrane molecule expressed by thymocytes, mature T cells, and neurons in CD90.2-expressing mouse strains (BALB/c, CBA, C3H, C57BL/6, C58, SJL, etc.).
Cells from CD90.1-expressing strains (PL, AKR) do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes, ≤0.05 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.
Titrate antibody for optimal performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample to antibody cocktails or single-color controls (100 µL cell sample, 10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology based on novel nucleic acid dye structures for engineered fluorescence with minimized spillover and spread.

Excitation / Emission / Laser:

• 552 nm / 663 nm / 561 nm (Yellow Laser)

Strain-dependent behavior:
NovaFluor Yellow 660 may stain B cells non-specifically in SJL mice. No non-specific staining observed in BALB/c or C57BL/6 mice.

Target Information

CD90.2 (Thy-1) antigen is a GPI-linked glycoprotein of the Immunoglobulin superfamily.
Expressed in murine T cells, thymocytes, neural cells, Kupffer's cells, fibroblasts.
Plays roles in cell-cell interactions during synaptogenesis and other neural events.
CD90.2 is an 18 kDa plasma membrane antigen, expressed in hematopoietic cells, neurons, connective tissue, fibroblast and stromal cell lines, and tumor endothelial cells.
In mice, expressed on all thymocytes and peripheral T lymphocytes.

For Research Use Only. Not for use in diagnostic procedures.

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