Thermo Fisher Scientific K-Ras Polyclonal Antibody
K-Ras 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC/IF에 사용 가능. Human과 Mouse에 반응하며 Affinity Chromatography로 정제됨. 합성 펩타이드 면역원 기반으로 제작되어 높은 특이성과 재현성을 제공.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.2–1 µg/mL | View 2 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | – | View 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide directed towards the N-terminal of human KRAS (aa 2–51) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS with 2% sucrose |
| Contains | 0.09% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2609818 |
Product Specific Information
Peptide sequence:
TEYKLVVVGA GGVGKSALTI QLIQNHFVDE YDPTIEDSYR KQVVIDGETC
Sequence homology:
Cow: 100%
Dog: 100%
Guinea Pig: 100%
Horse: 100%
Human: 100%
Mouse: 100%
Rabbit: 100%
Rat: 100%
Yeast: 100%
Zebrafish: 100%
Target Information
K-RAS (GTPase KRas) belongs to the Ras oncogene family. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. It plays an important role in the regulation of cell proliferation and contributes to oncogenic events by inducing transcriptional silencing of tumor suppressor genes in colorectal cancer cells in a ZNF304-dependent manner.
Mutations in the gene are associated with leukemia (acute myelogenous, juvenile myelomonocytic), Noonan syndrome, gastric cancer, and cardiofaciocutaneous syndrome 2.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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