Thermo Fisher Scientific Spiramycin Polyclonal Antibody

Applications

Tested Dilution

Publications

ELISA (ELISA)

5-10 µg/mL

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Spiramycin-BTG

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

purified

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1090852

Product Specific Information

Working Concentration: 2.5 µg/mL. Sensitivity: 10 ng/mL Spiramycin produces 77% inhibition in a competitive ELISA employing Spiramycin polyclonal antibody.

Target Information

Aminoglycosides are a family of bacterial antibiotics that are used in the treatment of specific bacterial infections. They display a concentration dependent killing action and are active against a wide range of aerobic Gram-negative bacilli. Aminoglycosides are molecules that are comprised of an amino group and a sugar group. They operate by inhibiting the bacteria from producing proteins vital to its growth. More specifically, they bind to the bacterial 30S ribosomal subunit where they prevent the translocation of the peptidyl-tRNA from the A-site to the P-site, subsequently giving rise to a misreading of mRNA resulting in the inhibition of protein synthesis. This consequently results in a disruption to the integrity of the bacterial cell membrane . In addition to their use to prevent bacterial infection, aminoglycosides have been used as growth promoters in food producing animals.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.