Thermo Fisher Scientific Eco72I (PmlI) (10 U/μL)

Thermo Fisher Scientific Eco72I (PmlI) (10 U/μL)

Thermo Scientific™ Eco72I (PmlI) 제한효소는 CAC^GTG 서열을 인식하며, Tango 버퍼에서 37°C에서 최적의 절단 활성을 보입니다.
이 효소는 10 U/μL 농도로 제공되며, CpG 메틸화에 민감하지만 Dam 및 Dcm 메틸화에는 민감하지 않습니다.

인식 서열 및 절단 위치

제품 설명

Thermo Scientific Eco72I (PmlI) restriction enzyme recognizes CAC^GTG sites and cuts best at 37°C in Tango buffer.
See Reaction Conditions for Restriction Enzymes for enzyme activity, double digestion conditions, and heat inactivation details.
Also available as a FastDigest enzyme for rapid DNA digestion.
Isoschizomers: AcvI, BbrPI, PmaCI, PmlI, PspCI

Thermo Scientific conventional restriction endonucleases are high-quality enzymes optimized for the Five Buffer System.
The universal Tango buffer is included for convenience in double digestions.
All enzymes exhibit 100% activity under recommended conditions.
Buffers contain premixed BSA to enhance enzyme stability and bind DNA contaminants.

특징 (Features)

• Superior quality—stringent QC and industry-leading manufacturing
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

응용 분야 (Applications)

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• RFLP analysis
• SNP genotyping

주의사항 (Note)

Eco57I requires only Mg²⁺ for activity but is stimulated by S-adenosylmethionine (0.01 mM increases activity 100-fold).
High glycerol (>5%), pH >8.0, or excess enzyme may cause star activity.
Eco57I may remain associated with cleaved DNA, causing band shifts during electrophoresis.
To avoid atypical patterns, use 6X DNA Loading Dye & SDS Solution or heat the digested DNA in SDS before electrophoresis.
For methylation sensitivity, refer to product specifications.

사양 (Specifications)