Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), PerCP-Cyanine5.5, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
• View 71 publications

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 (~35 kDa) is expressed as a disulfide-linked dimer on lymphocyte surfaces. Activation of T and B cells rapidly upregulates CD69 expression, indicating its role in lymphocyte development and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Activated splenocytes
• Recommended Usage: ≤0.5 µg/test in 100 µL final volume (10⁵–10⁸ cells/test)
• Excitation: 488 nm
• Emission: 695 nm
• Laser: Blue Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It contains one or two N-linked oligosaccharides and is present on activated platelets. In normal peripheral blood, CD69 expression varies among cells and plays a role in lymphocyte signal transduction and proliferation. It may also transmit signals in NK cells and platelets. Multiple transcript variants exist due to alternative splicing.
Diseases associated with CD69 dysfunction include coccidioidomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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