ELK Biotechnology CA VB rabbit pAb
CA5B 단백질을 인식하는 토끼 다클론 항체로, WB, IHC, IF, ELISA에 사용 가능. 미토콘드리아 내 탄산탈수효소 VB 검출에 적합. 사람, 생쥐, 랫트 시료 반응. -20°C에서 1년 보관 가능.
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제품명
CA VB rabbit pAb
대체 명칭
CA5B; Carbonic anhydrase 5B; mitochondrial; Carbonate dehydratase VB; Carbonic anhydrase VB; CA-VB
적용 분야 (Applications)
- WB (Western Blot)
- IHC (Immunohistochemistry)
- IF (Immunofluorescence)
- ELISA
권장 희석 배수 (Recommended Dilutions)
| 실험 방법 | 희석 배수 |
|---|---|
| Western Blot | 1/500 - 1/2000 |
| Immunohistochemistry | 1/100 - 1/300 |
| Immunofluorescence | 1/200 - 1/1000 |
| ELISA | 1/10000 |
| 기타 | Not yet tested in other applications. |
면역원 (Immunogen)
The antiserum was produced against synthesized peptide derived from human CA5B.
AA range: 241-290
보관 및 안정성 (Storage)
- Host: Rabbit
- Storage condition: -20°C / 1 year
항체 특성 (Antibody Properties)
| 항목 | 내용 |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| 농도 (Concentration) | 1 mg/ml |
| 관찰된 밴드 (Observed Band) | 38 kD |
유전자 정보 (Gene Information)
| 항목 | 내용 |
|---|---|
| GeneID (Human) | 11238 |
| Swiss-Prot No. | Q9Y2D0 |
세포 내 위치 (Cellular Localization)
Mitochondrion
반응 종 (Species Reactivity)
Human; Mouse; Rat
배경 (Background)
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in various biological processes including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid.
CA VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. It has a wider tissue distribution than CA VA, which is restricted to the liver. These differences suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles.
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