Thermo Fisher Scientific CD3e (T-Cell Marker) Monoclonal Antibody (B-B12)

Applications

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1–2 µg/mL

Flow Cytometry (Flow)

• Tested Dilution: 1–2 µg/10⁶ cells

Product Specifications

Target Information

The CD3 subunit complex is crucial for transducing antigen-recognition signals into the cytoplasm of T cells and regulating the surface expression of the TCR complex.
T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of CD3 subunits (gamma, delta, epsilon, zeta), which are structurally related members of the immunoglobulin superfamily encoded by genes on human chromosome 11.

CD3 subunits have long cytoplasmic tails that associate with signal transduction molecules via a double tyrosine-based motif.
CD3 may play roles in TCR-induced growth arrest, cell survival, and proliferation.
The CD3 antigen is expressed on 68–82% of normal peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells in the cerebellum, but not on B or NK cells.
Decreased T lymphocyte percentages may be observed in some autoimmune diseases.
Genes encoding CD3 epsilon, gamma, and delta are located on chromosome 11, and defects are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.