Thermo Fisher Scientific PureLink Expi Endotoxin-Free Mega Plasmid Purification Kit

Invitrogen™ PureLink™ Expi Endotoxin-Free Mega Plasmid Purification Kit

The PureLink Expi Endotoxin-Free Mega Plasmid Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange membrane. In as little as 2 hours, purify up to 5 mg of high-quality plasmid DNA suitable for advanced transfection applications such as transfection of sensitive cell lines or genome engineering.

주요 특징

• 간단하고 빠른 프로토콜: 전체 정제 과정이 약 2시간 내에 완료
• 고수율: 최대 5 mg의 고품질 플라스미드 DNA 정제 가능
• 엔도톡신-프리 DNA: 0.1 EU/μg 미만, 민감한 응용에 이상적
• 고순도: 단백질, RNA, 게놈 DNA 오염이 극히 낮음
• 호환성: ExpiCHO 및 Expi293 발현 시스템과 완전 호환
• 엔도톡신-프리 구성품: 엘루션 버퍼, 물, 플라스미드 저장 튜브 모두 엔도톡신-프리

Simple and Fast Protocol

Unlike other anion-exchange-based endotoxin-free plasmid purification systems, this kit utilizes centrifugation- or vacuum-assisted filtration for lysate clarification and subsequent plasmid DNA purification. There is no waiting for gravity-flow columns, and the entire purification typically takes about 2 hours (including the final precipitation step).

Endotoxin-Free Plasmid

Purify endotoxin-free (<0.1 EU/μg) plasmid ideal for sensitive applications such as transfection of primary cells, genome engineering, gene therapy research, or plasmid vaccine studies in vivo.

Compatibility with ExpiCHO and Expi293 Expression Systems

Plasmid purified using this kit is fully compatible with Gibco ExpiCHO and Expi293 transient mammalian expression systems.

Superior Quality with Endotoxin-Free Components

The kit includes endotoxin-free elution buffer, water, and plasmid storage tubes to ensure purity and reliability.

제품 사양