Invitrogen™

Zero Blunt™ TOPO™ PCR Cloning Kit, with pCR™-Blunt II-TOPO™ Vector, One Shot™ TOP10 Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit

Zero Blunt™ TOPO™ PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified자세히 알아보기

Zero Blunt™ TOPO™ PCR Cloning Kits offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kits include linearized and topoisomerase I-activated pCR™-Blunt II-TOPO™ Vector for 5-minute benchtop ligations without ligase (1). The pCR™-Blunt II-TOPO™ Vector also features:

• ccdB gene for positive selection (2)
• Eco_R I sites flanking the PCR product insertion site for easy excision of inserts
• Kanamycin and Zeocin™ resistance genes for your choice of selection in _E. coli
• SP6 promoter/primer site for in vitro RNA transcription and sequencing
• M13 forward and reverse primer sites for sequencing or PCR screening

Selected Zero Blunt™ TOPO™ PCR Cloning Kits are available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO™-cloned inserts for downstream analysis.

사양

박테리아 또는 효모 균주TOP10

세포 유형Chemically Competent

클로닝 방법Blunt TOPO™

형식Kit

제품라인One Shot™

제품 유형PCR Cloning Kit

프로모터SP6

수량25 Reactions

벡터Blunt DNA Cloning Vectors

Unit SizeEach