Thermo Fisher Scientific Transferrin Polyclonal Antibody, Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

Immunohistochemistry (IHC)

1:1,000-1:5,000

-

ELISA (ELISA)

1:20,000-1:150,000

-

Immunoprecipitation (IP)

1:2,000-1:10,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Transferrin [Human Serum] if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Transferrin,uniProtId:P02787-1,ncbiNodeId:9606,antigenRange:1-698,antigenLength:698,antigenImageFileName:209-4634-0100_Transferrin_P02787-1_Rabbit.svg,antigenImageFileNamePDP:209-4634-0100_Transferrin_P02787-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

1 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/mL BSA

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Transferrin Antibody, Biotin Conjugated, is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Transferrin [Human Serum]. Cross reactivities against Transferrin from other sources may occur but have not been specifically determined.

Target Information

Transferrins (Tf) are an approximately 80kDa blood plasma glycoprotein synthesized by the liver. They are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Transferrin is the primary blood iron transport protein and under normal conditions; approximately one-third of total blood transferrin contains bound iron. Measurement of blood transferrin levels can be used as an indicator for blood iron-carrying capacity and abnormalities of iron metabolism such as anaemia, iron overload and haemochromatosis.Serum transferrin may also have a further role in stimulating cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.