Invitrogen™

SYBR GreenER™ qPCR SuperMix Universal

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we`ve taken the best of SYBR GreenER qPCR SuperMix and added additional capabilities for your gene expression analysis.

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SYBR™ GreenER™ qPCR SuperMixes Universal incorporate the latest high-performance technology to provide the most reliable gene expression data on a variety of platforms, including ABI 7500, Corbett Rotor-Gene™, MJ Chromo4™ and Opticon™, and Stratagene instruments. The SYBR™ GreenER™ system is specially formulated to offer the best sensitivity, specificity, and reproducibility with:
• A novel dsDNA-binding dye - exhibits a brighter signal for increased sensitivity and less PCR inhibition than original SYBR™ Green I, while using the same instrument filters and settings
• An optimized buffer system - improves sensitivity and provides excellent long-term stability
• Uracil DNA glycosylase - reduces carryover contamination in qPCR

Novel Formulation Provides More Reliable Data
The SYBR™ GreenER™ qPCR SuperMix kits include a new dsDNA-binding dye that produces a brighter signal and significantly less PCR inhibition, for improved qPCR performance over a broad dynamic range.

Consistent Specificity and Low-Copy Detection
The SYBR™ GreenER™ qPCR reagent system minimizes the formation of nonspecific products, including primer-dimers, for greater accuracy across a wide range of targets. The reliability of the SYBR™ GreenER™ qPCR reagent system also translates to greater sensitivity. With SYBR™ GreenER™ qPCR SuperMix, you can consistently detect fewer than 10 copies of a target in genomic DNA.

Eight cDNA targets and eight genomic DNA targets were amplified from 1 ng of cDNA and 6 pg of gDNA, respectively, on the ABI PRISM™ 7900HT Sequence Detection System using the SYBR™ GreenER™ qPCR SuperMix for ABI PRISM™ instruments. For all 16 targets, detection was positive in the presence of template but negative for all no-template controls.

For Research Use Only. Not for use in diagnostics procedures.

사양

검출 방법SYBR

형식Tube

GC-Rich PCR PerformanceHigh

PCR 방법qPCR

반응 속도Standard

용도(장비)7500 System, Stratagene Mx4000, Stratagene Mx3000P, Stratagene Mx3005P, MJ Chromo4, MJ Opticon

제품라인Platinum™, SYBR™ GreenER™

제품 유형Real Time PCR SYBR Master Mix

수량100 reactions

배송 조건Dry Ice

충분100 Reactions

용도(애플리케이션)Gene Expression

농도2X

반응 수100 Reactions

샘플 종류DNA (Genomic), cDNA

Unit SizeEach