Thermo Fisher Scientific WTIP Polyclonal Antibody
WTIP 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC에 사용 가능. 인간 시료에 반응하며, Protein A 및 항원 친화 크로마토그래피로 정제됨. PBS buffer에 보관, 연구용 전용 시약.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:50 | - |
| Immunocytochemistry (ICC/IF) | 1:10–1:50 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between 243–271 amino acids of human WTIP |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.35 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2633750 |
Product Specific Information
Predicted to react with mouse based on sequence homology.
Target Information
Adapter or scaffold protein involved in multiple cellular processes such as cell fate determination, cytoskeletal organization, gene transcription repression, cell-cell adhesion, differentiation, proliferation, and migration.
Positively regulates microRNA (miRNA)-mediated gene silencing and negatively regulates the Hippo signaling pathway by antagonizing YAP1 phosphorylation.
Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription.
Functions as a hypoxic regulator by linking prolyl hydroxylases and VHL for efficient HIF1A degradation.
In podocytes, contributes to actin regulation and foot process architecture, shuttling into the nucleus during injury to repress WT1-dependent transcription and alter gene expression.
Also involved in basal body organization, cilia growth, and positioning.
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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