Thermo Fisher Scientific Ki67 Monoclonal Antibody (OTI16E9), TrueMAB
인간 Ki-67 단백질을 인식하는 단클론 항체로, 세포 증식 마커 연구에 적합합니다. IHC(P)에서 1:100 희석으로 사용 가능하며, 고순도 친화 크로마토그래피 정제 제품입니다. PBS, BSA, 글리세롤 완충액에 보관되며, 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI16E9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1160–1493 of human MKI67 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly during late G1, S, G2, and M phases. It contains a forkhead-associated (FHA) domain that mediates association with euchromatin at the perichromosomal layer, centromeric heterochromatin, and nucleolus.
Ki-67 exhibits a cell cycle-dependent localization pattern — perinucleolar in G1, nuclear matrix in G2, and chromosomal during M phase.
As Ki-67 is absent in G0 cells but increases from G1 through mitosis, it serves as a reliable proliferation marker. Ki-67 antibodies are widely used to determine the growth fraction of neoplastic cells, with prognostic value comparable to the tritiated thymidine-labeling index. A low Ki-67 index correlates with histologically low-grade tumors.
Ki-67 is routinely utilized as a neuronal marker for cell cycling and proliferation studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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