Thermo Fisher Scientific Human CASK, GST Tag Recombinant Protein

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

Baculovirus

Amino acid sequence

1-570

Tag

GST-tag

Molecular weight

91 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris HCl, pH 7.5, with 10mM glutathione, 0.1mM PMSF, 0.1mM EDTA, 150mM NaCl, 0.25mM DTT, 25% glycerol

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

Recombinant human CASK (1-570) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen™ Eu Kinase Binding Assay

Protein Form: Recombinant, Fragment

Assay Entry: Biochemical binding/kinetic

Target Information

CASK is a calcium/calmodulin-dependent serine protein kinase which is a MAGUK (membrane-associated guanylate kinase) protein family member. CASK is a scaffold protein and the encoded protein is located at synapses in the brain. CASK associates with FG syndrome 4, mental retardation, microcephaly with pontine and cerebellar hypoplasia, and a form of X-linked mental retardation. CASK functions as a cytoskeletal membrane scaffold that coordinates signal transduction pathways within the cortical cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.