Thermo Fisher Scientific UBE2E3 Monoclonal Antibody (OTI7E8)
Thermo Fisher Scientific의 UBE2E3 Monoclonal Antibody (OTI7E8)는 인간, 마우스 등 다양한 종에 반응하는 단클론 항체입니다. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 사용 가능하며, 고순도 친화 크로마토그래피 정제 및 안정적 PBS buffer에 보관됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7E8 |
| Immunogen | Full length human recombinant protein of UBE2E3 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2723755 |
Target Information
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates.
- The first step requires ATP-dependent activation of the Ub C-terminus and assembly of multi-Ub chains by the Ub-activating enzyme (E1).
- The Ub chain is conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex.
- The Ub-ligase (E3) catalyzes the transfer of Ub from E2 to the appropriate protein substrate.
UBE2E3, also known as UBCH9, catalyzes the covalent attachment of ubiquitin to other proteins and is involved in regulation of transepithelial sodium transport in renal cells. It may also play a role in cell growth arrest. The UBE2E3 protein shuttles between the cytoplasm and nucleus in an IPO11-dependent manner. It is ubiquitously expressed at low levels and highly expressed in skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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