Thermo Fisher Scientific HLA-B Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Purity is >95% by SDS-PAGE.

Target Information

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells.

The heavy chain is approximately 45 kDa and its gene contains 8 exons:

• Exon 1: leader peptide
• Exons 2 and 3: alpha1 and alpha2 domains (peptide binding)
• Exon 4: alpha3 domain
• Exon 5: transmembrane region
• Exons 6 and 7: cytoplasmic tail

Polymorphisms within exon 2 and exon 3 determine peptide binding specificity of each class I molecule. Typing for these polymorphisms is routinely performed for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.

HLA and MHC antibodies are crucial in Immunopeptidomics, aiding in the identification and characterization of neoantigens through high-performance liquid chromatography coupled with tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.