Thermo Fisher Scientific ACAP2 Polyclonal Antibody
Western blot 및 Immunocytochemistry에 적합한 Rabbit Polyclonal ACAP2 항체. Human, Mouse, Rat 등 다양한 종에 반응. 합성 펩타이드 면역원 기반으로 제작되었으며, 액상 형태로 1 mg/mL 농도 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ACAP2 (Accession Q15057), corresponding to amino acid residues G746–F778 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2899444 |
Product Specific Information
Antibody detects endogenous levels of total ACAP2.
Target Information
ACAP2 is a transport carrier belonging to the Centaurin family with three ANK repeats, an Arf-GAP domain, a BAR domain, and a PH domain.
It functions as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) with robust constitutive ARF6 GAP activity, stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid, but has little activity toward ARF1.
ACAP2 controls the return of ARF to the inactive GDP-bound state. Along with ACAP1, it is targeted by phosphatidylinositol (3,4,3) triphosphate, inhibiting the formation of PDGF-induced dorsal membrane ruffles in NIH3T3 fibroblasts when overexpressed.
ACAP2 is phosphorylated upon DNA damage, likely by ATM or ATR, and is widely expressed with the highest levels in lung tissue.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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