Thermo Fisher Scientific KLF15 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-2.5 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

2-10 µg/mL

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide region of human KLF15.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2132418

Product Specific Information

Recommended positive controls: HepG2.

Predicted reactivity: Cow: 86%; Dog: 86%; Guinea Pig: 85%; Horse: 92%; Mouse: 79%; Pig: 86%; Rabbit: 93%; Rat: 86%.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

KLF15 is a nuclear protein belonging to the Sp1 C2H2-type zinc-finger protein family with three C2H2-type zinc fingers. A transcriptional activator, KLF15 binds to the GA element of the CLCNKA promoter and may help to regulate the strict tissue and nephron segment-specific expression of CLCNKA and CLCNKB channel genes. It plays an essential role in adipogenesis in cells through its regulation of PPARG expression. Reports suggest that KLF15 is an inhibitor of cardiac hypertrophy and functions in a pathway, regulating the cardiac response to hemodynamic stress, and regulates mitochondrial acetyl-CoA synthase expression in skeletal muscle. KLF15 may also act as a transcriptional repressor of the rhodopsin and IRBP promoters in the retina, and is a possible participant in repression of photoreceptor-specific gene expression in nonphotoreceptor cells. It is highly expressed in liver, followed by heart, skeletal muscle, and kidney but not expressed in bone marrow or lymphoid tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.