Thermo Fisher Scientific TRIP12 Polyclonal Antibody
TRIP12 단백질을 인식하는 Rabbit Polyclonal Antibody로 인간 시료에 반응합니다. IHC, ICC/IF 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. PBS 기반 버퍼에 40% 글리세롤 포함, 4°C 또는 -20°C에서 안정적으로 보관 가능합니다.
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Thermo Fisher Scientific TRIP12 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,500–1:5,000 | - |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human TRIP12. Recombinant protein control fragment (Product #RP-96151). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2648954 |
Product Specific Information
Immunogen sequence:
AMCKEIIPTS EFINSKLTAK ANRQLQDPLV IMTGNIPTWL TELGKTCPFF FPFDTRQMLF YVTAFDRDRA MQRLLDTNPE INQSDSQD
Highest antigen sequence identity to the following orthologs:
- Mouse: 100%
- Rat: 100%
Target Information
E3 ubiquitin-protein ligase involved in the ubiquitin fusion degradation (UFD) pathway and regulation of DNA repair. Mediates ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A, a tumor suppressor required for p53 activation under oncogenic stress. In cancer cells, TRIP12 and p19ARF/ARF are separated in different compartments, preventing degradation. Also catalyzes ubiquitination of NAE1 and SMARCE1. Acts as a key regulator of DNA damage response by suppressing RNF168, preventing excessive accumulation of ubiquitinated chromatin at damaged chromosomes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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