Thermo Fisher Scientific IDH3A Polyclonal Antibody
Thermo Fisher Scientific의 IDH3A Polyclonal Antibody는 인간, 생쥐, 랫드에 반응하며 Western blot, IHC, ICC/IF에 적합합니다. 항원 친화 크로마토그래피로 정제된 액상 항체로, 0.84 mg/mL 농도이며 연구용으로 사용됩니다.
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Thermo Fisher Scientific IDH3A Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–293 of Human IDH3A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.84 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545468 |
Product Specific Information
- Recommended positive controls: HepG2, mouse brain, rat brain
- Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (91%), Xenopus laevis (92%), Chicken (94%), Rhesus Monkey (99%), Bovine (97%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one utilizing NAD(+) and the other NADP(+).
Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent enzymes localized to the mitochondrial matrix, and two NADP(+)-dependent enzymes (one mitochondrial, one cytosolic).
NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein described here is the alpha subunit of one NAD(+)-dependent isozyme.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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