Takara 5X PrimePath Probe qPCR Kit, GPR

The 5X PrimePath Probe qPCR Kit, GPR is ideal for sensitive pathogen detection via probe-based qPCR (using the 5′ nuclease method). The increased, 5X concentration of the master mix frees up reaction space in your qPCR-based pathogen detection assays; this allows you to add more of your sample, which improves the limit of detection, or to add additional primers/probes to develop multiplex assays.

The 5X PrimePath Probe qPCR Kit, GPR is ideal for sensitive pathogen detection via probe-based qPCR (using the 5′ nuclease method). The increased, 5X concentration of the master mix frees up reaction space in your qPCR-based pathogen detection assays; this allows you to add more of your sample, which improves the limit of detection, or to add additional primers/probes to develop multiplex assays.

The kit contains our efficient and highly specific hot-start PCR enzyme, TaKaRa Taq HS, in an optimized reaction buffer. Simply add a DNA sample, primers, and probes to the master mix to get the reaction started. The entire protocol, from setup to detection, takes less than one hour.

The 5X PrimePath Probe qPCR Kit, GPR is a general purpose reagent (GPR) labeled “For Laboratory Use” and is manufactured in a facility certified to ISO 13485. Every lot is functionally tested to ensure lot-to-lot reproducibility and consistent qPCR performance across a large dynamic range.

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Overview

• Increase the limit of detection for your qPCR-based pathogen detection assays—no preamplification needed
• Develop successful multiplex assays with increased reaction space for multiple primer/probe sets
• Go from reaction setup to detection in less than one hour
• Ensure high reproducibility and consistent performance across reactions with a general purpose reagent labeled “For Laboratory Use”

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Applications

• Diagnostic assay development
• Pathogen detection (viruses and/or bacteria)
• Multiplex real-time PCR

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.