Invitrogen™

ElectroMAX™ DH12S Cells

ElectroMAX DH12S Cells, a derivative of DH10B cells, are suitable for transformation by electroporation. They may be used for production자세히 알아보기

ElectroMAX DH12S Cells, a derivative of DH10B cells, are suitable for transformation by electroporation. They may be used for production of single-stranded (ss)DNA and in procedures requiring high transformation efficiencies, such as generation of cDNA, genomic, and subtractive cDNA libraries.

Benefits of ElectroMAS DH12S Competent Cells
• Maximize yield of transformants using small amounts of DNA
• High-yield plasmid production
• Ability to create representative genomic libraries

Optimized strain for ssDNA production
The DH12S strain is endA+, which allows the production of ssDNA with less contamination of double-stranded (ds)DNA. The lacIq on the F´ episome allows for regulatable expression from the lac promoter. Most E. coli strains used in cloning genomic DNA have intact the systems that restrict DNA containing methylated cytosine and adenine residues (coded by mcr_A, _mcr_B, _mcr_C, and _mrr genes). Such wild type systems have been eliminated in the DH12S strain, allowing the construction of more representative genomic libraries and use in more efficient plasmid rescue procedures. M13KO7 helper phage is supplied for the production of ssDNA and together with DH12S cells is utilized in antibody discovery workflows (e.g., screening Ab libraries using panning method).

ElectroMAX DH12S Cells offer:
• >1 x 1010 transformants/μg efficiency with electroporation for unsurpassed cloning results
• A unique genotype to support production of exceptionally clean single stranded DNA
• Stable F´ episome to eliminate the need for growth on minimal media
• Better sequence representation eliminating mcr_A, _mcr_BC, _mrr, _hsd_RMS restriction systems
• Blue/white screening of recombinant clones due to _lac_ZΔM15
• Ready-to-use M13KO7 helper phage for single stranded DNA production

Note: A High-voltage electroporation apparatus is required.

Genotype
φ80Δl_acZ_ΔM15 mcr_A Δ(_mrr-hsd_RMS-_mcr_BC) _ara_D139 Δ(_ara-leu)7697 Δ(_lac_X74 _gal_U _gal_K _rps_L(StrR) _nup_G _rec_A1 / F′ [_pro_AB+_lac_IqZΔM15 _Tn_10(TetR)]

Genetic marker descriptions

Find the strain and format that fits your needs
DH10B Cells, the parent strain of DH12S, are available in both electrocompetent and chemically competent formats.
MAX Efficiency DH5αF´IQ Competent Cells may be used for ssDNA production and come with M13mp18 RF DNA control and are chemically competent cells.

사양

제품 유형Electrocompetent Cells

에프에피솜 포함Yes

플라스미드 품질 개선No

메틸화 DNA 클로닝Yes (mcrA)

형질전환 효율 수준High Efficiency (>1 x 109 cfu/μg)

항생제 내성 박테리아Yes (Streptamycin, Tetracycline)

불안정 DNA 클로닝Not suitable for cloning unstable DNA

블루/화이트 스크리닝Yes (lacZΔM15)

고처리량 호환성Low

플라스미드May be used for plasmids >20 kb

비메틸화 DNA 준비No

재조합 감소Yes (recA1)

배송 조건Dry Ice

T1 Phage - 저항성(tonA)No

종E. coli (K12)

프로모터Laclq

형식Tube

제품라인ElectroMAX™

수량5 x 100 μL

Unit SizeEach