
Thermo Fisher Scientific Ubiquitin Polyclonal Antibody
Ubiquitin 단백질 검출용 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC 등 다양한 응용에 적합. 고순도, 2 mg/mL 농도 액상 형태. 모든 종에 반응하며 연구용으로 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:10–1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | All, Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between 36–66 amino acids of human Ubiquitin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Size-exclusion - Dialysis, Ammonium sulfate precipitation |
| Storage buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2269673 |
Product Specific Information
This antibody is predicted to react with bovine, chicken, canine, equine, hamster, mouse, porcine, non-human primate, rat, rabbit, Xenopus, and yeast based on sequence homology.
Target Information
Ubiquitin is a highly conserved protein that plays a major role in targeting cellular proteins for degradation by the 26S proteasome. It is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. The gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S ribosomal subunit and contains C4-type zinc finger domains located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. Ribosomal proteins L40 and S30 are also synthesized as fusion proteins with ubiquitin or ubiquitin-like proteins. Multiple alternatively spliced transcript variants encoding the same proteins have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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