Thermo Fisher Scientific TID1 Polyclonal Antibody
TID1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF, IP에 사용 가능하며, Human에 반응. 항원 친화 크로마토그래피로 정제, PBS 기반 저장 버퍼 포함. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | – |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
| Immunoprecipitation (IP) | – | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–446 of Human TID1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.46 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2544702 |
Product Specific Information
- Recommended positive controls: HepG2, HCT116
- Predicted reactivity: Mouse (87%), Rat (87%), Bovine (89%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
TID1 is a human homolog of the Drosophila tumor suppressor lethal (2) tumorous imaginal discs, l(2)tid, and encodes two mitochondrial matrix-localized splice variants of human Tid-1 designated hTid-1S and hTid-1L. These proteins are conserved members of the DnaJ family, acting as cochaperones for mitochondrial Hsp70. They contain a conserved J domain that binds to Hsp70 chaperones and activates their ATPase activity.
Expression of hTid-1L increases apoptosis induced by DNA-damaging agents such as mitomycin-C and TNF-α, while J-domain mutants can modulate apoptotic activity differently between hTid-1L and hTid-1S. These variants influence cytochrome c release and caspase 3 activation, suggesting that their relative abundance may regulate mitochondrial apoptotic signaling.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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