Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (Gi18), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

3-30 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

30 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

30 µg/mL

-

Immunocytochemistry (ICC/IF)

5 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-Dependent

-

ELISA (ELISA)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

Gi18

Immunogen

Rec. Human CD31 (PECAM-1)

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Biotin
• FITC
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2-7.4

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10596359

Product Specific Information

Description: The antibody BMS137 recognizes platelet endothelial cell adhesion molecule 1 (PECAM-1) also known as CD31, a transmembrane glycoprotein with a molecular weight of approximately 130 kDa, depending on the degree of glycosylation. PECAM-1 is constitutively expressed on all vascular cells and has provided a useful immunohistochemical marker of blood vessels, particularly in the setting of angiogenesis. It has also been found on platelets, monocytes, neutrophils and CD8+ T cells. Bone marrow stem cells and transformed cell lines of the myeloid and megakaryocytic lineage also express PECAM-1. Interestingly, PECAM-1 was also detected on human, mouse and rat solid tumor lines.

Recent studies suggest a role for PECAM-1 in the inflammatory process and leukocyte-endothelial interaction. PECAM-1 is an early and sensitive marker for tumor-induced angiogenesis. Several data have suggested that PECAM-1 may be involved in the process of angiogenesis in a developing vertebrate embryo as well as during metastases formation.

Applications Reported: This BMS137 antibody has been reported for use in ELISA, western blotting under non-reduced conditions, cell staining followed by both FACS and flow cytometric analysis, immunohistochemical staining of both frozen and FFPE tissues.

Applications Tested: This BMS137 antibody has been tested by western blot analysis and immunohistochemistry on human FFPE tissue using both low and high pH antigen retrieval at 30 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: >95%.

Target Information

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.