Thermo Fisher Scientific EpCAM (CD326) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.01-3 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

5-20 µg/mL

-

Product Specifications

Species Reactivity

Pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein Epithelial Cell Adhesion Molecule. The antigen corresponds to amino acid range 25-265 of the target protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EpCAM (CD326),uniProtId:Q75QW1-1,ncbiNodeId:9821,antigenRange:25-265,antigenLength:314,antigenImageFileName:PA5-119097_EpCAM_CD326_Q75QW1-1_Rabbit.svg,antigenImageFileNamePDP:PA5-119097_EpCAM_CD326_Q75QW1-1_Rabbit_PDP.jpeg,sortOrder:4}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2903597

Target Information

Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.