Thermo Fisher Scientific DKK3 Polyclonal Antibody
DKK3 단백질을 표적하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC(F), ICC/IF 등 다양한 응용에 적합. 재조합 인간 DKK3(aa 72–350) 항원을 면역원으로 사용하며, 1 mg/mL 농도의 액상 형태로 제공. 4°C 단기, -20°C 장기 보관 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 72–350 of Human DKK3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2547999 |
Product Specific Information
- Recommended positive controls: 293T, A431, Jurkat, Raji
- Predicted reactivity: Mouse (84%), Rat (81%), Dog (86%), Pig (86%), Rhesus Monkey (97%), Bovine (88%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and two conserved cysteine-rich domains, separated by a linker region containing 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 12 amino acids in DKK3, compared to 50–55 amino acids in DKK1, DKK2, and DKK4. All DKKs have several potential sites for cleavage by furin-type proteases.
Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization shows highest expression in mouse brain, eye, and heart.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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