Thermo Fisher Scientific PTP1B Polyclonal Antibody
Rabbit polyclonal antibody targeting human PTP1B (PTPN1). Validated for WB, IHC(P), and ICC/IF. Affinity-purified, liquid format, 0.2 mg/mL concentration. Supplied in PBS with 40% glycerol, for research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human PTPN1 (amino acids 268–406). Recombinant protein control fragment available (Product #RP-90585). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789620 |
Product Specific Information
Immunogen sequence:
RFSYLAVIEG AKFIMGDSSV QDQWKELSHE DLEPPPEHIP PPPRPPKRIL EPHNGKCREF FPNHQWVKEE TQEDKDCPIK EEKGSPLNAA PYGIESMSQD TEVRSRVVGG SLRGAQAASP AKGEPSLPEK DEDHALSYW
Target Information
PTP1B (PTPN1) is a tyrosine-protein phosphatase that regulates the endoplasmic reticulum unfolded protein response by dephosphorylating EIF2AK3/PERK, thereby inactivating its kinase activity. It participates in CKII- and p60c-src-induced signaling cascades and modulates EFNA5-EPHA3 signaling, influencing cell organization and repulsion. PTP1B also regulates hepatocyte growth factor receptor (MET) signaling through dephosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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