Thermo Fisher Scientific FTO Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

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ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 327-504 of human FTO (NP_0010739011) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:FTO,uniProtId:Q9C0B1-1,ncbiNodeId:9606,antigenRange:327-504,antigenLength:505,antigenImageFileName:PA5-89791_FTO_Q9C0B1-1_Rabbit.svg,antigenImageFileNamePDP:PA5-89791_FTO_Q9C0B1-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.49 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2805778

Product Specific Information

Positive Samples: 293T; Cellular Location: Nucleus, Nucleus speckle

Immunogen sequence: STGTLDYILQ RCQLALQNVC DDVDNDDVSL KSFEPAVLKQ GEEIHNEVEF EWLRQFWFQG NRYRKCTDWW CQPMAQLEAL WKKMEGVTNA VLHEVKREGL PVEQRNEILT AILASLTARQ NLRREWHARC QSRIARTLPA DQKPECRPYW EKDDASMPLP FDLTDIVSEL RGQLLEAK

Target Information

FTO is a novel protein and a member of the non-heme dioxygenase (Fe(II)-and 2-oxoglutarate-dependent dioxygenases) superfamily. Amino acid conservation patterns support this hypothesis and indicates that both 2-oxoglutarate and iron should be important for FTO function. Though not much is yet known about FTO, it is thought that the protein may somehow modify the activity of genes involved in metabolism and fat storage, which in turn may influence a person`s risk of obesity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.