Thermo Fisher Scientific CD27 Monoclonal Antibody (O323), NovaFluor Yellow 610, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.8 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 615 / 737 / 805
• Brilliant Violet 421 / 480 / 605 / 711
• eFluor 450
• FITC
• NovaFluor Blue 610-70S / 660-120S / 690 / 725 / 760 / 800
• NovaFluor Red 700 / 725 / 755
• NovaFluor UV 765
• NovaFluor Violet 690
• NovaFluor Yellow 590 / 660 / 810
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation available

Product Specific Information

The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and most mature T cells, and is upregulated upon T-cell stimulation. CD27 binds to CD70, playing a key role in T cell–B cell interactions.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometry using normal human peripheral blood cells. Recommended use: 5 µL (0.8 µg) per test in a final volume of 100 µL. Cell number: 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample to antibody cocktails before combining with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample.

NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures engineered for optimal fluorescent signatures with reduced spillover and spread.

Excitation: 552 nm
Emission: 612 nm
Laser: 561 nm (Yellow)

Target Information

CD27 is a 50 kDa member of the TNF receptor superfamily, including CD40 and CD30. It is primarily expressed on lymphocytes and plays a role in regulating cell proliferation and apoptosis. CD27 is found as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells, and a subset of B cells. Upon T-cell activation, CD27 expression increases, and soluble CD27 (sCD27) can be detected in plasma, urine, or spinal fluid—serving as a prognostic marker in B cell malignancies. CD27-binding protein (SIVA) is involved in CD27-mediated apoptosis.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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